Novel Purine Derivative ITH15004 Facilitates Exocytosis through a Mitochondrial Calcium-Mediated Mechanism

Upon depolarization of chromaffin cells (CCs), a prompt release of catecholamines occurs. This event is triggered by a subplasmalemmal high-Ca2+ microdomain (HCMD) generated by Ca2+ entry through nearby voltage-activated calcium channels. HCMD is efficiently cleared by local mitochondria that avidly take up Ca2+ through their uniporter (MICU), then released back to the cytosol through mitochondrial Na+/Ca2+ exchanger (MNCX). We found that newly synthesized derivative ITH15004 facilitated the release of catecholamines triggered from high K+-depolarized bovine CCs. Such effect seemed to be due to regulation of mitochondrial Ca2+ circulation because: (i) FCCP-potentiated secretory responses decay was prevented by ITH15004; (ii) combination of FCCP and ITH15004 exerted additive secretion potentiation; (iii) such additive potentiation was dissipated by the MICU blocker ruthenium red (RR) or the MNCX blocker CGP37157 (CGP); (iv) combination of FCCP and ITH15004 produced both additive augmentation of cytosolic Ca2+ concentrations ([Ca2+]c) K+-challenged BCCs, and (v) non-inactivated [Ca2+]c transient when exposed to RR or CGP. On pharmacological grounds, data suggest that ITH15004 facilitates exocytosis by acting on mitochondria-controlled Ca2+ handling during K+ depolarization. These observations clearly show that ITH15004 is a novel pharmacological tool to study the role of mitochondria in the regulation of the bioenergetics and exocytosis in excitable cells.


Introduction
By acting on receptors, ion channels or intracellular signalling pathways, various endogenous neurotransmitters and exogenous compounds have been implicated in the positive or negative regulation of the exocytotic release of catecholamines from adrenal medulla chromaffin cells (CCs) [1]. In this physiological process, Ca 2+ ions play a central role in triggering the stimulus-secretion coupling process [2]. During CC activity, cytosolic Ca 2+ concentrations ([Ca 2+ ] c ) may undergo brisk sudden changes from its basal concentrations of around 0.1 µM to tens micromolar at exocytosis subplasmalemmal sites, giving rise to vesicle fusion and fast exocytosis. Milder 0.5-1 µM [Ca 2+ ] c elevations, as a result of Ca 2+ redistribution, occur at inner cytosolic areas to regulate pre-exocytotic steps, such as Ca 2+ -dependent vesicle transport to reload the secretory machinery [1,[3][4][5].
Those Ca 2+ microdomains (CMDs) are originated and tightly regulated by endogenous messengers including Ca 2+ itself and various pharmacological ligands acting on Ca 2+ entry through voltage-activated calcium channels (VACCs), their redistribution into the endoplasmic reticulum (ER) Ca 2+ store and mitochondria, and its subsequent release into the cytosol from those CC organelles. For instance, by stimulating the release of ER Ca 2+ in bovine CCs (BCCs), we have previously shown that the Na + ,K + -ATPase pump blocker ouabain [6] and, more recently, the vesicle dopamine transporter blocker tetrabenazine (TBZ) [7] facilitated the depolarizing-evoked catecholamine release through the mobilization of ER Ca 2+ . These findings could have interest in the context of safety issues with the handling of these drugs in clinical sets (i.e., ouabain and other digitalis drugs have been, and are being, used in cardiac failure and atrial fibrillation, while TBZ is currently used in the treatment of dystonic movements in Huntington's disease (HD)). Facilitation of catecholamine release by these drugs may also distort the flight-or-fight response during stressful conflicts [8]. Eventually, this facilitation could find some therapeutic projection in dysautonomic diseases, certain types of shock or in some disorders that benefit from excessive dopamine release, such as the hyperactivity and attention deficit syndrome in children [9]. In this context, drugs that facilitate the release of adrenal catecholamines have interest from efficacy and safety points of view.
In our laboratory, we culminated the design and synthesis of new families of nonnucleotide purine derivatives. This program aimed at searching new blockers of the ionotropic purinergic receptor P2X7 (P2X7R). As this cation channel is emerging as a relevant gatekeeper of neuroinflammation [10], a P2X7R blocker could mitigate such process and displays activity in limiting the progression of neurodegenerative diseases; this was proven to be the case in the SOD1 G93A mouse model of amyotrophic lateral sclerosis (ALS) [11]. Among the newly synthesized compounds, we selected ITH15004 as a mild potent P2X7R blocker that mitigates the ATP-induced IL-1β release from macrophages and the [Ca 2+ ] c transients elicited by B Z ATP stimulation of human P2X7R-expressing HEK293 cells [12]. When exploring its general pharmacology, we found that compound ITH15004 ( Figure 1) facilitated the release of catecholamines from isolated BCCs stimulated with K + depolarizing pulses. Such facilitation seems to be due to alterations of Ca 2+ handling by mitochondria, which in BCCs gives rise to a notable potentiation of exocytosis [13]. Here, we report the pharmacological effects of compound ITH15004 on catecholamine release and Ca 2+ handling by BCCs. It seems that the compound may somehow interfere with mitochondrial Ca 2+ movements to augment the [Ca 2+ ] c available for exocytosis during CC depolarization.

Effects of Compound ITH15004 on Catecholamine Release
After placing a BCCs batch into the perfusion microchamber, real-time recording of catecholamine release was monitored at 37 • C for a 5 min period, in order to reach a stable baseline. Then, at 1 min intervals, cells were sequentially challenged with 5 s pulses of a high K + solution (i.e., 35 mM K + , low Na + , the so-called 35K + solution). Figure 2A shows an original recording of secretion responses elicited by each of the 35K + pulses (P1 to P22) applied to each cell batch. In this experiment, the initial peak secretion amounted to 200 nA equivalents of catecholamines, that gradually decreased to reach 45.7% of P1 at P22. Pooled data from 14 cell batches from six different cell cultures show that the decay of exocytosis along the experiment reached 45.7 ± 2.2% of P1 at P22 (black square curve in Figure 2C). , original record of secretory responses elicited by 35K + challenges in cells that were exposed to compound ITH15004 during the time period indicated by the top horizontal bar. Insets in A and B are calibration bars (secretion of catecholamines in nA versus time). (C), normalized averaged secretion expressed as percentage of P6 in each individual experiment (ordinate), expressing the time course (abscissa) of secretory spike amplitude in control cells and cells exposed to ITH15004 (top horizontal bar, pulses P7 to P16). Data are means ± SEM of the number of experiments (n) and the different cell cultures (N) shown in parentheses (n,N). (D), pooled data on the concentration-response effects of ITH15004 in enhancing the secretory responses to 35K + pulses. Data are normalized as percentage of P16 and correspond to the last pulse P16 in both control cells (100% ordinate) and cells exposed to increasing concentrations of ITH15004 (abscissa) (points marked with an ellipse in panel (C)). They are means ± SEM of the number of experiments (n) and separate cell cultures (N) shown in parenthesis (n,N). * p < 0.05, ** p < 0.01, *** p < 0.001 with respect to control.
In the experiment shown in Figure 2B, cells were similarly challenged with 35K + pulses, displaying the typical decay of secretory responses during P1 to P6. However, upon cell exposure to 1 µM of ITH15004, the secretory peak responses underwent two notable changes: firstly, their gradual decay stopped; secondly, the responses gradually increased above the initial P1 value, to reach a plateau at P12, which slowly began to decay again upon compound washout. This behaviour is more clearly observed in the normalized averaged secretory responses (% P6) of the black circle curve in Figure 2C. The gradual augmented secretion is sustained in a plateau in the presence of ITH15004. Normalizing the response to P16 (the last 35K + pulse in the presence of the compound), augmented secretion was estimated to be concentration-dependent at 0.3-1 µM; at higher concentrations (3-10 µM), facilitation of secretion was still present, but to a lesser extent. This suggests that two distinct mechanisms of action could be present at lower and higher ITH15004 concentrations, as argued in the Discussion.

Effects of ITH15004 on Secretion from Cells with Their Endoplasmic Reticulum Calcium Store Depleted
We have previously used a mixture of 10 mM caffeine, 1 µM ryanodine and 1 µM thapsigargin (the so-called CRT mixture) to deplete quickly, drastically, and irreversibly the ER Ca 2+ store of CCs [14]. As ER Ca 2+ mobilisation is involved in the potentiation by ouabain of secretion triggered by K + in BCCs [6] and tetrabenazine [7], a possibility exists that ITH15004 was acting in a similar way. Thus, we tested the effects of the compound on secretion in BCCs with their ER Ca 2+ store depleted. Figure 3A shows an original trace from an experiment that started with six 35K + control 5 s pulses. Subsequently, CRT was introduced in the perifusion fluid during the decay phase of P6, with an elevated baseline that was due to rapid ER Ca 2+ mobilisation and depletion, and the stable subsequent lower spikes in the presence of CRT. Upon CRT washout the secretory spikes remained stable. In the original trace of Figure 3B, a similar protocol was used although ITH15004 (1 µM) was applied during the exposure of CCs to CRT. A progressive augmentation of secretion occurred. These responses decayed upon washout of both CRT and ITH15004. This increase of secretion, which is better observed in Figure 3C, where pooled results from eight experiments are plotted, was similar in the absence or the presence of CRT when secretion is normalized to control response in P16 (ellipse in panel C) secretion was 91.4 ± 6.2% in the presence of CRT and 149.4 ± 8.4% in the presence of CRT+ITH15004 (p < 0.001) ( Figure 3D). In summary, facilitation of secretion by ITH15004 was still present in CCs with their ER Ca 2+ store depleted and non-functional.

Effects of ITH15004 on Secretion upon the Pharmacological Interference with Mitocondrial Calcium Handling
Upon depolarization of BCCs, Ca 2+ entry through VACCs creates a large submembrane Ca 2+ transient that triggers exocytosis. Mitochondria contribute much to shape and clear such transient in these cells [13,15]. Mitochondria take up large amounts of Ca 2+ through their Ca 2+ uniporter (MICU), thanks to the existing large proton gradient [16][17][18]. Therefore, if such proton gradient is dissipated by a protonophore, mitochondria cannot contribute to clear up the large submembrane Ca 2+ transient generated nearby subplasmalemmal exocytotic sites. Consequently, longer and larger Ca 2+ transients occur, which give rise to higher exocytotic responses during K + depolarization of BCCs [13,19]. Hence, experiments were done to find out whether compound ITH15004 modified somehow the effects of a protonophore on exocytosis.  Figure 4A shows experiments where a batch of BCCs were challenged with sequential 5 s pulses of 35K + at 1 min intervals. The typical gradually declining secretory responses are observed during P1 to P6. At P7, the protonophore FCCP (3 µM) was introduced causing a drastic 3.5-fold potentiation of the 35K + evoked response, which quickly returned to a mildly elevated baseline respect to the initial one. The response remained increased in P8 and then decayed progressively to reach a plateau at a mildly higher level than the pre-protonophore level of secretion. Then, secretion was further depressed upon FCCP washout at P16, but it started a slow recovery during P21-P22. In the experiment shown in Figure 4B, a similar protocol was followed. However, when the response was decaying in the presence of FCCP, ITH15004 at 1 µM was added. Secretion decay stopped, and the responses remained stable in a plateau from pulses P12 to P16, in which both compounds were present. Washout of both FCCP and ITH15004 resulted in a fast-brisk fall of secretion (P17 to P19), which started again to recover in the following 35K + pulses. When added on top of FCCP, the effects of ITH15004 are better represented in the plot of pooled data shown in Figure 4C. For comparison, the time course of the decay of the secretion responses to repeated 35K + challenging is also plotted (black squares). The decaying phase of the FCCP curve (black circles) is drastically stopped upon addition of ITH15004. The averaged normalized data on the effects of ITH15004 on secretion in the presence of FCCP, are represented in the bar graph of Figure 4D. At 0.3 µM, the compound did not modify the secretory response in the presence of FCCP, but at 1 and 3 µM ITH15004 increased such response by 72.9 ± 11.1% and 75.9 ± 10.6%, respectively. At 10 µM, however, the augmentation was milder (19.3 ± 5.6%). We were interested in determining how ITH15004 affected the 35K + responses when simultaneously added together with FCCP. Figure 5A represents an original recording showing a similar pronounced potentiation of secretion to that previously observed with FCCP alone ( Figure 4A). However, an important difference with the experiment of Figure 4A arose: the amplitudes of the 35K + secretory responses slightly decreased at P7 and P8 but were maintained high and non-inactivated in the subsequent P9 to P16 pulses. Upon washout of the two compounds, responses decayed drastically and quickly to very low levels, showing once more a tendency to recover in subsequent stimuli. We also inquired whether the blockade of the mitochondrial Na + /Ca 2+ exchanger (MNCX) with CGP37157 (1 µM) would affect the responses to 35K + in the presence of combined FCCP+ITH15004. The simultaneous cell exposure to the three compounds produced the typical initial potentiation of secretion (P7 and P8). However, surprisingly, the presence of CGP37157 counteracted the effect of ITH15004 and provoked a quick decay of secretory responses to reach tiny amplitudes. Upon washout of the three compounds, the responses initiated a progressive recovery ( Figure 5B). Again, the time courses of the secretion responses are plotted in Figure 5C with pooled data. The decay of secretion in cells treated with the combined three compounds is consistently higher compared to the responses of cells exposed to only FCCP plus ITH15004. This was also the case for cell exposed to combined FCCP, ITH15004 and ruthenium red (RR, 1 µM), an MICU blocker. Plotting of the relative responses normalised to P16 ( Figure 5D) shows the potentiation of secretion by FCCP plus ITH15004, and its drastic blockade by compound CGP37157 and by RR.

Effects of ITH15004 on Barium Currents
As K + -elicited secretion fully depends on Ca 2+ entry through VACCs, we explored whether compound ITH15004 affected the whole-cell inward calcium currents triggered by depolarization of voltage-clamped BCCs. Facilitation of exocytosis by ITH15004 could be due to an augmented Ca 2+ entry through VACCs, similarly to the dihydropyridine BayK8644 [20]. Figure 6A shows a family of current traces (2 mM Ba 2+ as divalent cation carrier, I Ba ), generated by sequential 50 ms depolarizing pulses to −10 mV, applied from a holding potential of −80 mV. The initial peak current in each trace corresponds to inward Na + currents (I Na ), which is followed by the typical non-inactivating I Ba (Control trace). This current (478 pA amplitude), was reduced to 392 pA in the presence of ITH15004 (1 µM) for 1 min. Added on top of the compound, ω-conotoxin GVIA (GVIA) at 1 µM, a N-type VACCs blocker further reduced I Ba to 294 pA. Nifedipine at 10 µM, a L-type VACCs-blocking dihydropyridine, caused a further current blockade (to about 233 pA). Partial blockade of I Ba corroborates the multiple VACC subtypes expressed by BCCs [4]. Washout of the compounds only partially recovered I Ba , as GVIA causes irreversible blockade of N-type VACCs [21]. Figure 6B better shows the time course of I Ba blockade elicited by the cumulative exposure to the three compounds. The remaining unblocked I Ba (around 50%) is due to the P/Q type of VACCs, that carries about half the total current in BBCs [4]. Pooled averaged data from 12 cells are plotted in Figure 6C. Relative blockade of I Ba accounted for 19 ± 1.2%, 20.8 ± 0.8% and 17.9 ± 1.3%, for ITH15004, GVIA, and nifedipine, respectively.

Effects of ITH15004 on the Cytosolic Calcium Transients Elicited by K +
At each moment of cell activation, the size and shape of the [Ca 2+ ] c depend not only on Ca 2+ entry, but also on its redistribution into the ER [22] and mitochondria [13]. Thus, we performed experiments to inquire how (if at all) compound ITH15004 could affect those intracellular Ca 2+ movements. This was tested in cell populations of BCCs loaded with the Ca 2+ -sensitive fluorescent dye fluo-4-AM.
After recording for a minute to estimate basal [Ca 2+ ] c fluorescence, cells were challenged with 35K + pulses (one pulse per well in 96-well plates). Changes of [Ca 2+ ] c were expressed as arbitrary fluorescence units (AFU) [7]. Figure 7A displays a family of [Ca 2+ ] c transient curves in the absence (control) and presence of increasing ITH15004 concentrations. At 0.3, 1, and 3 µM the compound augmented the [Ca 2+ ] c peaks elicited by 35K + . At 10 µM the [Ca 2+ ] c transient was also augmented but to a lesser extent. This is more clearly shown in Figure 7C where averaged data from 9 cell batches are plotted. The concentrations of 0.3, 1 and 3 µM augmented peak [Ca 2+ ] c by 71.6 ± 11.1%, 101.2 ± 9.8% and 87.6 ± 10.1%, respectively. At 10 µM, the [Ca 2+ ] c increase was 40.8 ± 22.8 but it did not reach the level of statistical significance when compared to control. Of interest was the observation that baseline [Ca 2+ ] c was elevated by ITH15004, as shown in the original full traces shown in Figure 7B (arrow Basal). Dots represent 35K + addition. Baseline elevations (recording of traces just before applying the 35K + pulse) are averaged in Figure 7D. Basal fluorescence is significantly increased in cells pre-exposed to increasing concentrations of ITH15004. This augmented basal [Ca 2+ ] c is compatible with an effect of ITH15004 on Ca 2+ mobilisation from an intracellular organelle. This was explored next.

Effects of ITH15004 on K + Elicited [Ca 2+ ] c Elevations in Cells with their ER Calcium Store Depleted
The potential effect of ITH15004 on ER Ca 2+ movements was explored in cells with their ER Ca 2+ store irreversibly depleted by CRT. The effects of the compound on [Ca 2+ ] c were explored with experimental protocols like those presented in Figure 7.
As shown in Figure 8A, CRT itself augmented the [Ca 2+ ] c signals control response. Added on top of CRT, ITH15004 further augments the response ( Figure 8A) Figure 8C) . Interestingly, CRT caused the expected elevation of basal Ca 2+ (Figure 8A,D) and, moreover, addition of ITH15004 to CRT did not further increase basal [Ca 2+ ] c at all the concentrations ( Figure 8D).

Effects of ITH15004 on 35K + Elicited [Ca 2+ ] c Elevations upon Interfering with Mitochondrial Calcium Handling
In BCCs, mitochondria are a major contributor to the size and shaping of Ca 2+ elevations elicited by 35K + depolarization [13]. Thus, we explored whether ITH15004 affected the 35K + -evoked [Ca 2+ ] c elevations in fluo-4-loaded BCCs.
First, we tested whether protonophore FCCP at 1 µM, which dissipates the mitochondrial membrane potential and interrupt their ability to take up and retain Ca 2+ from the cytosol, affected the elevations of [Ca 2+ ] c elicited by ITH15004. Figure 9A shows that FCCP augmented the 35K + induced [Ca 2+ ] c transients, as expected. Added on top of FCCP, ITH15004 still caused further elevations of [Ca 2+ ] c in a concentration-dependent manner ( Figure 9A,B).Added FCCP in combination with the MICU blocker RR at 1 µM, the [Ca 2+ ] c elevation was 107% (a 42% higher than when FCCP was added alone; comparing panels B and D of Figure 9). Under these conditions, ITH15004 added on top of FCCP+RR did not increase further the 35K + -elicited [Ca 2+ ] c transient ( Figure 9C,D). This indicates that MICU seems to be involved in the augmentation of [Ca 2+ ] c elevations elicited by ITH15004.
Another experiment explored the possible involvement of MNCX in the effects of ITH15004 on [Ca 2+ ] c . Thus, 1 µM of the MNCX blocker CGP37157 [13] was used in combination with FCCP. Combined FCCP+CGP elicited a 60% increase of [Ca 2+ ] c , a lesser potentiation compared with the augmentation produced by FCCP+RR (comparing panels D and F of Figure 9). Added on top of FCCP+CGP, ITH15004 did not increase further the 35K + -evoked [Ca 2+ ] c elevations ( Figure 9E,F).
Finally, we investigated whether compound JNJ-47965567 (JNJ), a potent blocker of P2X7Rs, was mimicking somehow the effects of ITH15004 on mitochondrial Ca 2+ handling.

Discussion
The central finding of this investigation is the facilitation of the K + -elicited exocytotic release of catecholamines from BCCs by the novel purine derivative compound ITH15004 ( Figure 2B). As the K + secretory response strictly depends on Ca 2+ ions [23], it seems plausible to link those effects to some influence of ITH15004 on the handling of Ca 2+ by BCCs. The secretory responses of BCCs upon their challenging with sequential 35K + pulses applied at regular intervals underwent a progressive decline (Figure 2A). Decay of spike amplitudes may have various components affecting either Ca 2+ handling by the cell and/or vesicle transport and priming after each 35K + pulse, whereby the secretory machinery is depleted of a ready-release vesicle pool [3]. In BCCs, we have previously suggested that the lower [Ca 2+ ] c elevations required for vesicle transport and priming [24] is controlled by ER Ca 2+ fluxes [22,25] and/or by mitochondrial Ca 2+ movements [13,26]. As depletion of ER Ca 2+ with CRT did not affect the ability of ITH15004 to augment exocytosis ( Figure 3B), it seemed that this Ca 2+ store is not contributing to the effects of the compound. This was proven by an experiment monitoring the [Ca 2+ ] c transients elicited by K + ; upon ER Ca 2+ depletion with CRT, cells still responded with higher [Ca 2+ ] c transients in the presence of increasing concentrations of ITH15004 ( Figure 8B).
In sharp contrast, ITH15004 deeply affected the secretory responses under conditions of pharmacological manipulation of Ca 2+ handling by mitochondria. The drastic potentiation of secretion by FCCP is due to impaired Ca 2+ uptake (and hence of Ca 2+ circulation) in mitochondria [13]. The decay of those responses in the continued presence of FCCP ( Figure 4A) are likely dependent on mitochondrial deterioration of Ca 2+ -dependent ATP production [17,18] that is necessary to maintain exocytosis [3]. Compound ITH15004 exhibited two effects on these FCCP-potentiated secretory responses, namely slowing down of secretory decay (when added after FCCP) and full prevention of secretion decay (when added with FCCP). This strongly supports the view that ITH15004 is facilitating exocytosis by acting on Ca 2+ handling by mitochondria. This was proven through the monitoring of [Ca 2+ ] c elevations of basal [Ca 2+ ] c and [Ca 2+ ] c elevations triggered by 35K + . While ITH15004 did not exert major changes of [Ca 2+ ] c under conditions of ER Ca 2+ depletion, its effects on mitochondrial Ca 2+ were notorious. Thus, under cell exposure to FCCP when mitochondria are unable to trap cytosolic Ca 2+ because of the dissipation of the proton gradient and mitochondrial depolarization [17,18], ITH15004 was still capable of increasing the 35K + -induced [Ca 2+ ] c elevations, even above the levels previously augmented by FCCP alone. Furthermore, additional observations showed that the interruption of mitochondrial Ca 2+ circulation, either by RR (blockade of Ca 2+ influx) or CGP (blockade of Ca 2+ efflux), cancelled the augmentation of [Ca 2+ ] c elicited by ITH15004 in the presence of FCCP. This suggests two interesting mechanisms involved in ITH15004 effects. First, the compound requires active mitochondrial Ca 2+ circulation even in the presence of FCCP and is reinforced by ITH15004, that might contribute to maintain ATP synthesis. In so doing, it is plausible that ITH15004 is facilitating on the one hand, vesicle transport and priming of the secretory machinery by maintaining for longer time the elevated basal [Ca 2+ ] c during the resting periods in between the repeated 35K + pulses. On the other hand, through its effects in facilitating mitochondrial Ca 2+ circulation, ITH15004 may contribute to maintain ATP synthesis and hence, the ATP required for maintaining healthy secretory responses upon repeated stimuli of BCCs. How these effects are exactly exerted, and which are the mitochondrial targets on which ITH15004 is acting, is a matter of further investigation.
A last point deserves attention. As explained in the Introduction, compound ITH15004 was developed in the frame of a program searching for new purine derivatives with potential ability to block P2X7Rs. As CCs are known to express various subtypes of purinergic receptors [27], the possibility exists that the effects here described for ITH15004, a mildly potent P2X7R blocker with an IC 50 of 14 µM [12], could be mediated by those receptors. This did not seem to be the case for two reasons: (i) the effects here described on Ca 2+ handling and exocytosis, were maximally observed at 1-3 µM, concentrations that do not target P2X7Rs [12]; and (ii) potent P2X7R blocker JNJ47965567 (with a nanomolarranged IC 50 ), did not affect either Ca 2+ handling or exocytosis.
In conclusion, compound ITH15004 elicited both a mild potentiation of catecholamine secretory responses triggered by BCC depolarization and a maintenance of those enhanced responses for longer time. These effects are exerted by interfering with Ca 2+ handling by mitochondria. If this improvement is also exerted under mitochondrial deficits of Ca 2+ handling and ATP synthesis, compound ITH15004 may become a useful pharmacological tool to correct those deficits in, for instance, cell, tissue, and animal models of neurodegenerative diseases. In the light of the results here obtained, further investigation on cell and animal models of those diseases are warranted. Additionally, the precise target(s) that regulate mitochondrial Ca 2+ circulation, on which ITH15004 could act, is a subject worth of further investigation.

Preparation of Bovine Chromaffin Cell Cultures
BCCs were isolated from adrenal glands of adult cows following standard methods [28] with some modifications [29,30]. They were plated at a density of 5 × 10 6 cells per 5 mL of Dulbecco's modified Eagle's medium (DMEM) in 6 cm diameter Petri dishes (to study secretion), in 12 mm-diameter glass coverslips at a density of 5 × 10 4 cells (to study barium currents, I Ba ), or into 96-well black plates at a density of 2 × 10 5 cells per well, to monitor Ca 2+ signals. Experiments were performed 2-4 days after plating.

Real-Time Monitoring of Catecholamine Release Triggered by High-K+ Depolarizing Pulses
Cells were scrapped off carefully from the bottom of the Petri dish (5 × 10 6 cells per dish) with a rubber policeman and centrifuged at 100x g for 10 min. The cell pellet was resuspended in 200 µL of Krebs-HEPES solution (composition in mM: 144 NaCl, 5.9 KCl, 1.2 MgCl 2 , 11 glucose, 10 HEPES, 2 CaCl 2 , pH 7.4 adjusted with NaOH). Cells were introduced in a 100 µL microchamber, previously filled with a filter for cell retention, and perfused at a rate of 2 mL/min. The liquid flowing from the superfusion chamber reached an electrochemical detector (model CH-9100; Metrohm AG, Herisau, Switzerland) placed just at the outlet of the microchamber, which monitors in real time the amount of catecholamines secreted under the amperometry mode. The catecholamines were oxidized at +0.65 V and the oxidation current was recorded with a frequency of 2 Hz, to monitor the amount of total catecholamine secreted [31]. To stimulate the catecholamine secretion, a Krebs-Hepes solution containing KCl at 35 mM with isosmotic reduction of NaCl (35K + solution), was applied in 5 s pulses at 1 min regular intervals at 37 ºC. After each stimulation, we measured in real time the catecholamine release by amperometry [30][31][32]. Representative records of some experiments shown in this report were accomplished by importing the data obtained in ASCII format to the Origin 8.6 (Microcal) program.

Electrophysiological Recording
Barium currents were recorded using the whole-cell configuration of the patch-clamp technique [33]. Coverslips containing the cells were placed on an experimental chamber mounted on the stage of Nikon Diaphot inverted microscope. Cells were perifused at 22 ± 2 • C with a Tyrode solution containing (in mM): 137 NaCl, 1 MgCl 2 , 2 CaCl 2 , 10 HEPES, pH 7.4 (adjusted with NaOH). Once the patch membrane was ruptured and the whole cell configuration of the patch-clamp technique was established, the cell was locally, rapidly and constantly perfused with an extracellular solution of similar composition to the chamber solution, but containing 2 mM Ba 2+ to monitor I Ba . In this way, we obtained greater and less inactivating currents than if we used 2 mM of Ca 2+ . Moreover, 2 mM of Ba 2+ allows us to see the maximum effect of toxins in blocking the different VACC subtypes in CCs [34]. Cells were internally dialyzed with an intracellular solution containing (in mM): 100 Cs-glutamate, 14 EGTA, 20 TEA·Cl, 10 NaCl, 5 Mg·ATP, 0.3 Na·GTP, and 20 HEPES, pH 7.3 (adjusted with CsOH). For I Ba data acquisition, cells were voltage-clamped at −80 mV and step-depolarisations with 50 ms depolarising pulses were applied at 30-s intervals to minimise current rundown [35]. Data were acquired with a sampling frequency of 20 kHz using the PULSE 8.74 software (Heka Elektronic, Lambrecht, Germany). Linear leak and capacitive components were subtracted by using a P/4 protocol and series resistance was compensated by 80%. The data analysis was performed with Igor Pro (Wavemetrics, Lake Oswego, OR, USA) and PULSE programs (Heka Elektronik).

Monitoring of Cytosolic Calcium Levels
To monitor the changes of [Ca 2+ ] c , cells were plated at a density of 2 × 10 5 cells per well into 96-well black plates, and the experiments were performed 48 h later. Cells were loaded with a Krebs-HEPES solution (composition in mM: 144 NaCl, 5.9 KCl, 1.2 MgCl 2 , 11 glucose, 10 HEPES, 2 CaCl 2 , pH 7.4 adjusted with NaOH) with 10 µM fluo-4-AM and 0.2% pluronic acid. Cells were incubated for 45 min at 37 • C in the dark. After this incubation period, cells were washed twice with the Krebs-HEPES solution at room temperature in the dark. Changes in fluorescence (excitation 485 nm, emission 520 nm) were measured using a fluorescent plate reader (Fluostar; BMG Labtech, Offenburg, Germany). Basal fluorescence levels were monitored before adding the stimulation solution (35K+) with an automatic dispenser. After stimulation of the cells, changes in fluorescence were measured for 60 s. To normalize fluo-4 signals, responses from each well were calibrated by measuring maximum and minimum fluorescence values. At the end of each experiment, 5% Triton X-100 (F max ) was added, followed by 2 mM MnCl 2 (F min ). Data were calculated as a percentage of F max -F min .

Chemicals
The following chemicals were used: collagenase type I, FCCP (carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone), ruthenium red (RR), caffeine, ryanodine (Ry), thapsigargin, and CGP37157, were from Sigma/MercK (Madrid, Spain); Dulbecco's modified Eagle's medium (DMEM), bovine serum albumin fraction V, foetal calf serum, and antibiotics were from Gibco (Madrid, Spain). Fluo-4-AM from Life Technologies (Madrid, Spain). JNJ-47965567 was provided by Janssen Pharmaceutica NV (Beerse, Belgium). All the other chemicals used were reagent grade from Merck and Panreac Química (Madrid, Spain). FCCP, RR, Ry and Thapsigargin mother solutions (10 −2 M) were prepared in dimethyl sulfoxide (DMSO) and protected from light. Final drug concentrations were obtained by diluting the stock solutions directly into the extracellular solution. At these dilutions, solvents had no effect on the parameters studied.

Data Analysis
Data are presented as single examples to illustrate the experimental protocols and as averaged pooled data from several experiments, as means ± SEM of the number of different experiments done (number of cells, n) and different cultures used (N). Mathematical analyses were done using the GraphPad Prism software, version 5.01 (GraphPad Software Inc., La Jolla, CA, USA). Comparison between means of group data were performed by oneway analysis of variance (ANOVA) following by the Tukey's post hoc test when appropriate.
Funding: This work was supported by the European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie grant agreement no. 766124 and by Acciones Estratégicas en Salud, Proyectos de Investigación en Salud (ISCIII/FEDER, PI19/01724). We thank the continued support of Fundación Teófilo Hernando.

Institutional Review Board Statement:
The experiments here reported were done in primary cultures of CCs from adrenal glands obtained in a local slaughterhouse, where cow meat is prepared for human use. In spite of this, we declare that all experiments in our laboratories are always carried out in accordance with the ARRIVE guidelines established by the Spanish National Council on Laboratory Animal Care and the European Union directive for the Use of Animals in Scientific Research. The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of Universidad Autónoma de Madrid.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy.